Cover of: Storage of platelet concentrates following ultraviolet-B irradiation | Robert B. Johnson

Storage of platelet concentrates following ultraviolet-B irradiation

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Ultraviolet radiation, Blood plat
StatementRobert B. Johnson
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Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. As irradiation performed in a blood center or a hospital will probably be associated with a variable postirradiation delay before transfusion, the ability Storage of platelet concentrates following ultraviolet-B irradiation book store PCs after UVB irradiation becomes by:   Storage of buffy coat–derived PLT concentrates in PAS‐C up to 7 days seems safe regarding the risk of a positive blood culture.

If anything, transfusion of a single old PLT concentrate may decrease this risk the following by: 3. The Rationale and Use of Platelet Concentrates Irradiated With Ultraviolet-B Light Derwood H.

Pamphilon U LTRAVIOLET-B (UVB) irradiation has pro- found immunomodulatory effects and modi- fies a number of interactions within the immune system.~ These include impaired cutaneous im- mune responses such as contact hypersensitivity (CHS) and cellular antigen presentation by Cited by: Ultraviolet‐B (UV‐B) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization.

This study evaluated the effect of increasing doses of UV‐B radi Cited by:   Ultraviolet B (UV-B) irradiation abolishes lymphocyte function in mixed lymphocyte culture (MLC) and could theoretically prevent alloimmunization against HLA class I antigens.

We describe a system available in transfusion practice for such a treatment of platelet concentrates (PC).Cited by: 5. We have studied the response of platelets to a standardized osmotic stress following storage at either 4 C or 22 C.

Platelets In vitro changes in platelet function and metabolism following increasing doses of ultraviolet‐B irradiation, Transfusion, /j Extension of platelet concentrate storage by addition of sodium.

The quality of platelet concentrates is affected by the preparation methods and storage conditions. The quality of PCs can be evaluated by measuring platelet viability following transfusion. Such measurements are expensive and complicated to perform and in vitro methods are often used to evaluate PC quality as surrogate tests to in vivo viability.

Plt concentrates can be prepared from whole blood by using the two different methods outlined in Figure are referred to as the plt-rich plasma (PRP) method, which is used exclusively in the U.S., 1 and the buffy coat (BC) method 2 that is predominantly used in Europe (Canada is converting to this method).

Comparative studies have shown no difference in the quality of these plt. A single-donor platelet component containing less than 1 × 1 0 6 leucocytes. Technical information. Platelets, Apheresis, Leucocyte Depleted may be collected by a variety of apheresis systems using different protocols.

Since platelet yields may vary, each procedural protocol must be fully validated, documented and specifications set. During the preparation of Platelet Concentrates from Whole Blood, the blood should be: An important determinant of platelet viability following storage is: plasma pH.

During storage, the concentration of 2,3-diphophoglycerate(2,3-DPG) decreases in a unit of: Red Blood Cells. This percentage is significantly different from the 5 percent of patients in the group receiving ultraviolet B–irradiated, pooled platelet concentrates from random donors (UVB-PC) (P = ), 3.

Details Storage of platelet concentrates following ultraviolet-B irradiation EPUB

Abstract The magnitude of the pH change during platelet concentrate storage at 20–24°C in polyvinyl chloride containers is not determined solely by platelet count per cubic In vitro changes in platelet function and metabolism following increasing doses of ultraviolet‐B irradiation, Transfusion, /j Irradiation of platelet concentrates (PCs) with ultraviolet-B (UVB) light inactivates the contaminating white cells and might be an alternative to filtration for the prevention of alloimmunization.

The optimum liquid platelet storage temperature is 22–24 °C with continuous gentle agitation. Liquid PCs were originally stored at 4 °C until the late s when it was discovered that products stored at room temperature (22–24 °C) had longer in vivo survival and greater hemostatic efficacy than those stored at the colder temperature.

in platelet counts after just one day storage. Furthermore, UV irradiation has no impact on red blood cells or platelet morphology as was checked in the blood slides prepared under the microscope.

No abnormal red blood cells (e.g. spherical, sickle shaped, etc) or abnormal platelets (large platelets, etc) were observed after the irradiation. Ultraviolet B irradiation in the prevention of alloimmunization in a dog platelet transfusion model: UV-B to Prevent PLT Alloimmunization for seven days of storage, platelet concentrates are.

BACKGROUND: Bacterial contamination of platelet concentrates (PCs) is recognized as a serious threat to transfusion safety.

We developed a simple method for sterilization of PCs with short-wave ultraviolet light (UVC). The effects of treatment on the sterility of contaminated PCs and in vitro platelet (PLT) variables were evaluated. STUDY DESIGN AND METHODS: Plasma-reduced PCs were. Esther Pellham's 28 research works with citations and reads, including: Effects of storage time prolongation on in vivo and in vitro characteristics of 4°C-stored platelets: VIABILITY OF.

Liquid platelet concentrates were originally stored at 4° C until the late s when it was discovered that products stored at room temperature had longer in vivo survival and greater hemostatic efficacy than those stored at the colder temperature.

54–56 In fact, the shelf life of liquid platelets stored at 22° C was extended from 5 to 7. H C van Prooijen's 34 research works with citations and reads, including: Reactions and platelet increments after transfusion of platelet concentrates in plasma or an additive solution: A.

In vitro changes in platelet function and metabolism following increasing doses of ultraviolet-B irradiation. Johnson RB(1), Napychank PA, Murphy S, Snyder EL. Author information: (1)Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut.

The UV‐treated and control platelet concentrates were stored for an additional 5 days at 20–24 °C under standard blood bank conditions. Samples from platelets were taken for laboratory tests on days 0 (pretreatment), 3 and 5 of platelet storage using an aseptic.

Preparation of Platelet Concentrates. This study was approved by the Australian Red Cross Blood Service Human Research Ethics Committee. Whole blood units ( ± 45 ml) were collected (day 0) into bottom-and-top bags containing 63 ml citrate phosphate dextrose (Fresenius Kabi, Bad Homburg, Germany) and stored at 22 °C between collection and processing.

Platelet Preparations and Membrane Labeling.

Description Storage of platelet concentrates following ultraviolet-B irradiation EPUB

Platelet rich plasma was obtained by centrifugation of freshly drawn citrated blood from healthy individuals at g for 10 min or by taking 10 ml samples from PCs on day 1, day 7, and/or day 15 of storage. Subsequently, platelets were washed, and if indicated, labeled 20 min in PBS using µM PKH26 cell membrane dye (Sigma Aldrich, Zwijndrecht.

Holme S. () Storage of Platelet Concentrates in Plasma-Free Synthetic Media. In: Smit Sibinga C.T., Das P.C., Meryman H.T. (eds) Cryopreservation and low temperature biology in blood transfusion.

Developments in Hematology and Immunology, vol Patients should obtain a rise of 20, to 40, platelets/uL following 1 unit, Leukocyte reduction and ultraviolet B irradiation of platelets to prevent alloimmunization and refractoriness to platelet transfusions.

The Trial to Reduce Alloimmunization to Platelets Study Group Storage of platelet concentrates at 22°C. Use of leukocyte-depleted platelet concentrates for the prevention of refractoriness and primary HLA alloimmunization: a prospective, randomized trial.

Blood. ;77(1)–5. Epub /01/ 82 Leukocyte reduction and ultraviolet B irradiation of platelets to prevent alloimmunization and refractoriness to platelet transfusions.

Exposure of platelet concentrates (PCs) to ultraviolet B radiation (UVB) has been advocated as an alternative method for prevention of the onset of HLA sensitization in recipients.

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In this study, pooled PCs were irradiated in a Haemonetics UV irradiator (Haemonetics Corp, Braintree, MA) at a dose that did not induce platelet activation. Capon SM, Sacher RA, Deeg HJ. Effective ultraviolet irradiation of platelet concentrates in teflon bags.

Transfusion ; Fiebig E, Lane TA. Effects of storage and ultraviolet B irradiation on CDbearing antigen-presenting cells (monocytes) in platelet concentrates. Transfusion ;   BACKGROUND: Transfusion of platelet concentrates (PCs) is the basic treatment for severe platelet disorders.

PCs carry the risk of pathogen transmission, especially bacteria. Pathogen reduction (PR) by addition of photochemical reagents and irradiation with visible or ultraviolet (UV) light can significantly reduce this risk.

We present a novel approach for PR in PCs employing UVC light. Keywords: Hemostatic function, Transfusion efficacy, Apheresis platelet concentrates, Gamma irradiation, Thrombocytopenic patients Introduction Allogeneic platelet (PLT) transfusions are fundamental to the supportive care of patients with severe thrombocytopenia, but risks are associated with the use of any blood component [ 1, 2 ].unfiltered platelet concentrates, prestorage filtration prevented a rise in the IL-8 and TNF-alpha on day 3 of storage.

The concentration of IL-1 beta was lower than the minimum concentration value of the kit used for this purpose. IL-6 was detected only in 7 units of all filtered platelet concentrates on day 3.

Conclusion: These data indicate.Savage B: Platelet adenine nucleotide levels during room temperature storage of platelet concentrates.

TransfusionOwens M, Holme S, Heaton A, Sawyer S, Cardinali S: Posttransfusion recovery of function of 5-day stored platelet concentrates.